Lactic dehydrogenase is widely distributed in the various human organs and tissues.
The determination and separation of LDH isoenzymes proved to be of great diagnostic values in hepatocellular diseases, heart diseases, neoplastic diseases and in musculoskeletal disorders.
This report will describe the LDH isoenzyme activity alterations in comparison with serum-glutamic oxaioacetic transaminase and serum giutamic pyruvic transaminase in liver diseases and discuss the, usefullness of this procedure in the diagnosis and in following the clinical course of acute heptaisis.
Serum LDH isoenzyme fractions were separated by agar gel electrophoresis.
176 normal person, 23 patients with acute hepatitis, 10 patients with primary hepatoma and 10 patients with liver cirrhosis were studied.
The results were as follows:
1. Mean total LDH activity in normal subjects was 196.5±63.6 unit and there were no significant differences between sex and age groups.
2. The activities of isoenzymes in normal subjects were LDH2, LDH1, LDH3, in decreasing order snd there were no significant differences between LDH4 and LDH6.
3. In patients with acute hepatitis, the activity of LDH5 were markedly increased. Since SGOT and SGPT returned to normal range, it remained significantly high after 4th week of clinical onset.
4. In patients with liver cirrhosis, serum total LDH activity was within normal range, but the isoenzyme of LDH5 was slightly increased and it was related to the level of SGOT and SGPT.
5. In patients with primary hepatoma, serum total LDH activity was. 467.5±176.4 unit and the activity of LDH5 was greatly increased.
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